Research progress in culture, purification and isolation of synovial type A cells in vitro and their biological functions / 国际生物医学工程杂志

The temporomandibular joint (TMJ) is surrounded by a joint capsule, the smooth inner layer of which is called the synovial membrane. Synovium is involved in various intraarticular diseases, and it is a key area of joint disease. Synovial type-A cells are located in the lining layer of the synovial membrane, mostly on the side of the membrane close to the joint cavity. They have a strong phagocytic effect, and their main role is to remove the degradation products of the intra-articular and extracellular matrix. Various intra-articular diseases will affect the synovium, which is the key area of joint disease. The method of cell culture in vitro can effectively simulate the growth environment of cells in vivo and can accurately understand the effects of single and multiple factors on synovial cells, which has become a basic research method. In this review paper, the latest research progress in human temporomandibular joint type-A synoviocytes is reviewed from the aspects of cell origin, in vitro culture, cell purification, and cell biological function.

Research on automatic segmentation of tumor target of lung cancer in CBCT images by multimodal style transfer technology based on deep learning / 中华放射肿瘤学杂志

Objective:Due to the low contrast between tumors and surrounding tissues in CBCT images, this study was designed to propose an automatic segmentation method for central lung cancer in CBCT images.Methods:There are 221 patients with central lung cancer were recruited. Among them, 176 patients underwent CT localization and 45 patients underwent enhanced CT localization. The enhanced CT images were set as the lung window and mediastinal window, and elastic registration was performed with the first CBCT validation images to obtain the paired data set. The CBCT images could be transformed into" enhanced CT" under the lung window and mediastinal window by loading the paired data sets into cycleGAN network for style transformation. Finally, the transformed images were loaded into the UNET-attention network for deep learning of GTV. The results of segmentation were evaluated by Dice similarity coefficient (DSC), Hausdorff distance (HD) and the area under the receiver operating characteristic curve (AUC).Results:The contrast between tumors and surrounding tissues was significantly improved after style transformation. The DSC value of cycleGAN+ UNET-attention network was 0.78±0.05, HD value was 9.22±3.42 and AUC value was 0.864, respectively.Conclusion:The cycleGAN+ UNET-attention network can effectively segment central lung cancer in CBCT images.

Expression of γδT and CD4 + CD25 + T cells in peripheral blood of HIV-infected patients/AIDS patients and their correlation / 中华微生物学和免疫学杂志

Objective:To understand the expression levels of γδT cells and CD4 + CD25 + regulatory T cells in the peripheral blood of HIV-infected/AIDS patients, and explore the correlation and possible relationship between γδT cells and CD4 + CD25 + regulatory T cells in the progression of HIV infection/AIDS Mechanism. Methods:Immunofluorescent monoclonal antibody labeling technology and flow cytometry were used to detect 12 cases of AIDS, 19 cases of intermediate HIV infection, 15 cases of early HIV infection, and 30 cases of healthy physical examination in peripheral blood CD3 + T cells and CD4 + The expression levels of T cells, CD8 + T cells, γδT cells, CD4 + CD25 + regulatory T cells; Pearson was used to analyze the correlation between γδT cells and CD4 + CD25 + regulatory T cells. Results:The expression (Mean± SD, %) of γδT cells in the peripheral blood of the AIDS group, the mid-stage HIV infection group, the early HIV infection group and the healthy control group were: 4.46±1.37, 3.59±0.67, 3.12±0.33, 1.73±0.36, group The time ratio, F=6.091, P=0.018. The expression (Mean± SD, %) of CD4 + CD25 + regulatory T cells in the peripheral blood of the AIDS group, the mid-stage HIV infection group, the early HIV infection group, and the healthy control group were: 10.28±1.94, 7.37±1.03, 6.68±0.58, 4.03±0.82, ratio between groups, F=13.568, P=0.002. The comparison of γδT and CD4 + CD25 + regulatory T cells among the four groups is statistically significant, AIDS group>HIV mid-infection group>HIV early infection group>control group. In terms of correlation, γδT, CD4 + CD25 + regulatory T cells are negatively correlated with CD4 + T cells ( r value are -0.982, -0.712, P value are 0.001, 0.002, respectively), and positively correlated with CD8 + T cell counts ( r value are 0.873 , 0.809, P value are 0.000 and 0.000 respectively); γδT cells are positively correlated with CD4 + CD25 + regulatory T cells ( r=0.911, P=0.000). Conclusions:HIV infection induces the proliferation of γδT cells and CD4 + CD25 + regulatory T cells, both of which participate in the immune response caused by HIV infection. γδT cells are positively correlated with CD4 + CD25 + regulatory T cells, and the two may have a synergistic effect.

Effect of supernatant of mesenchymal stem cells on tumor necrosis factor-α and apoptosis protein caspase-3 in diabetic skin ulcer rat model / 国际生物医学工程杂志

Objective To investigate the supernatant of umbilical cord-derived mesenchymal stem cells (UCMSCs) on tumor necrosis factor-α(TNF-α) and apoptosis protein caspase-3 in diabetic rats model with skin ulcer. Methods 45 Sprague-Dawley rats were randomly divided into control group (acute wounds group), phosphate buffered saline (PBS) group and UCMSCs supernatant group. The diabetic rat model was constructed by injecting with alloxan by tail vein and feeding with high-fat diet. Diabetic skin ulcer (DSU) rat model was constructed by scratching a wound and infusing suspension of Staphylococcus aureus. In the control group, the diabetic rats (n=15) were scratched to form a wound and treated by tail vein injection of 100μl PBS. In the PBS group, DSU rats (n=15) were treated by tail vein injection of 100μl PBS, and then 100μl PBS was dropped at the ulcer site. In the UCMSCs supernatant group, freeze-dried powder of UCMSCs supernatant was dissolved in 200μl PBS, 100μl of which was injected into the tail vein of DSU rats (n=15), and other 100μl was dropped at the ulcer site. After 5 days of the treatments, the levels of serum TNF-αwere detected by radioimmunoassay method, and the expression of TNF-αand caspase-3 in the ulcer tissues of rats was detected by polymerase chain reaction and Western Blot. Results The levels of TNF-αin the PBS group [(35.9±3.7)μg/L] were significantly higher than that of the control group [(11.4±4.9)μg/L] and the UCMSCs group [(14.7±6.6)μg/L] (all P<0.05). The levels of mRNA and protein expression of TNF-αand caspase-3 in the UCMSCs group were significantly lower than those of the PBS group (all P<0.05), and have no significant differences with respect to those of the control group (all P>0.05). Conclusions UCMSCs supernatant treatments can effectively down-regulate the expression of TNF-αand caspase-3 in ulcer tissue of DSU rats, and play an anti-inflammatory and anti-apoptotic effect.

Plan robustness of craniospinal irradiation with VMAT / 国际生物医学工程杂志

Objective To compare the dosimetric characteristics of the methods of volumetric modulated arc therapy ( VMAT ) for craniospinal irradiation , and to compare their robustness to the field placement error . Methods Six patients receiving craniospinal irradiation were included. VMAT plans of each patient were optimized with overlap method and gradient-optimization method respectively using Pinnacle 9.8 VMAT treatment planning system. The length of the overlap region was set as 3 and 9 cm, respectively. Then the dose distributions under different VMAT programs were measured. Moreover, a 3 mm placement error was introduced, and the dose cold spot in the field junction region obtained by each plan was compared for robustness analysis. Results Under different overlapping lengths, the overlap method and the gradient optimization method both can optimize the VMAT plan that meeting the clinical requirements. In the field junction region, the dose distribution obtained by the overlap method was more uniform, and the difference in the uniformity index was statistically significant. When introducing a 3 mm placement error, the gradient optimization method obtained the most robust VMAT plan at 9 cm overlap length, and the overlap method could not obtained stabilized robust plan. Conclusions For the optimization of craniospinal irradiation VMAT plan, the commonly used overlap method can obtain a better dose distribution, but it can't improve robustness by increasing overlap length. However, using the gradient optimization method, the dose homogeneity in the field junction region is not good as the overlap method, but the plan robustness can be improved by increasing the overlap length.

Volumetric modulated arc therapy for nasopharyngeal carcinoma patients with poor compliance: setup error and expansion margin of target volume / 中华放射肿瘤学杂志

Objective To investigate the setup error for nasopharyngeal carcinoma (NPC) patients with poor compliance using kV cone-beam computed tomography,and to calculate the expansion margin from the clinical target volume (CTV) to planning target volume (PTV).Methods In 45 NPC patients from 2013 to 2015,the setup error,95％ confidence interval (CI)-1 for random error,and PTV-1 value were calculated.Moreover,in 16 NPC patients with poor compliance based on five verifications (random error not within 95％ CI-1),the setup error,95％ CI-2 for random error,and PTV-2 value were calculated.For the 16 special patients,PTV-1 and PTV-1 combined with PTV-2 were used to develop the plan-1 and plan-2,respectively.The dosimetric difference between plan-1 and plan-2 was evaluated.Results Both PTV-1 and PTV-2 had the largest expansion margin in the y direction.The CTV of plan-1 could not meet the requirement of the prescription dose after the setup error was introduced.Compared with plan-1,the V95％ and D95 values for the CTV of plan-2 were increased by 6.26％ and 4.43％,respectively.The D01 value was significantly larger in plan-2 than in plan-1 (P=0.005),which,however,met the clinical requirement.Conclusions In patients with poor compliance,the dose to target volume can be effectively elevated and the normal tissue can be spared from damage when PTV-1 combined with PTV-2 is selected as expansion margin.

Roles and mechanisms of γδT cells in bacterial infectious diseases / 中华微生物学和免疫学杂志

T cells are divided into two subsets,αβΤandγδT cells, according to the T-cell recep-tor ( TCR) expressed. γδT cells are a small minority of T cells and in contrast to αβΤ cells, they do not seem to require antigen processing and major-histocompatibility-complex ( MHC ) presentation of peptide epitopes. This group of T cells is usually much less common than αβT cells, but plays an important role in anti-infection, anti-tumor and immunoregulation. This review summarizes the production, development, dis-tribution, genetic characteristics, antigen recognition characteristics, biological and immunological functions of γδT cells as well as their unique roles and mechanisms in bacterial infectious diseases.

Proliferation activating effects of Mtb-Ag on γδT cells and its effects on expression of CD69 molecules inγδT cells / 国际生物医学工程杂志

Objective To activate and amplify γδT cells with low molecular peptide antigen of Mycobacterium tuberculosis low molecular peptide antigen (Mtb-Ag),and to investigate the expression of CD69 molecules on γδT cellular surface.Methods Healthy human peripheral blood mononuclear cells (PBMC) were obtained and separated,then positive cells were isolated by immuno-magnetic beads selection,and the proportion of γδT cells in the PBMCs was detected by fluorescent monoclonal TCR γδT-PE staining and flow cytometry.Expression of CD69 molecules in γδT cells was detected by γδ-PE/CD69 FITC double staining.Results The proportion of γδT cells was (4.9±1.85)％ in freshly obtained PBMC,(69.2±6.57)％ after 10 d of Mtb-Ag activation,and (99.3±8.92)％ after immuno-magnetic beads selection.The expression of CD69 molecules in γδT cells reached the peak (75.2％) at 24 h after initial Mtb-Ag stimulation,and reached the peak (72.0％) at 6 h after second stimulation.Conclusions MtbAg can specifically stimulate the proliferation of γδT cells in the PBMC.Both its initial and the second stimulation can specifically activate γδT cells.

Relationship between 3-d reconstruction of regenerated fibers and functional recovery after mastoid segment of facial nerve was repaired / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To explore the relationship between three-dimensional (3-D) reconstruction of regenerated fibers and functional recovery after mastoid segment of facial nerve was repaired with either end-to-end anastomosis or autogenous great auricular nerve grafting.@*METHOD@#Thirty healthy adult New Zealand white rabbits were randomly divided into two groups: one was end-to-end anastomosis group and another was autogenous great auricular nerve grafting group. Only mastoid segment of right facial nerve of each animal was dissected and the contralateral nerve was as a control. Electromyogram (EMG) were recorded from 5 animals of each group at the 30th d, 90th d and 120th d after operation. After that the nerves were extracted, fixed, decalcified and embedded in paraffin. Samples was sectioned serially at 6 microm and stained with special trichrome stain. All the imagines were imported into Mimics software to reconstruct the 3-D model.@*RESULT@#The significant differences were found in the regenerate fibers on 30 th d,and were found in amplitude of EMG on 30 th d and 90 th d. The image of 3-D reconstruction showed that the myelin sheath were thickening, connected from proximal to the distal gradually after repair.@*CONCLUSION@#The 3-D reconstruction of regenerated nerve fibers partly conformed to the functional recovery after facial nerve trunk was repaired. The functional recovery of facial nerve was related with both the quantity and the quality of regenerated nerve fibers.

Analysis of the surgery of 96 patients with hedge brain injury at frontotemporal bottom / 中国基层医药

Objective To explore the method and the clinical effects of surgical treatment for severe hedge brain injury at frontotemporal bottom. Methods 96 patients with severe hedge brain injury at frontotemporal bottom were scored by GOS before surgery. Then unilateral or bilateral craniotomy was carried out,via a large flap incision in the site of frontotemporal and parietal hair,and the blood clots within the surgical fieldand the inactivation brain tissue having been fell off were removed, and the contusion lesions of brain bottom were exposed, and the inactivation brain tissue was cleared. If the brain injury was serious ,the removal of frontal pole and temporal pole should be proper. Discretion to The removing or replacing the bone flap should be based on consideration of the circumstances. Six nonths or one year after injury,96 patients were scored by GOS again. Results There were 17 cases of grade Ⅰ(17.71%) ,11 cases of gradeⅡ (11.46%),13 cases of gradeⅢ (13.54%),21 cases of grade Ⅳ(21.86%),34 cases of grade Ⅴ (35.42%) in 96 patients. Conclusion Early surgical treatment of severe hedge brain injury at frontotemporal bottom could improve the cure rate and reduce the rate of disability.

Effect of imaging time on infarct size estimation after acute myocardial infarction using delayed contrast-enhancement magnetic resonance imaging / 中华急诊医学杂志

Objective To investigate whether timing of image acquisition influenced infarct size estimation using delayed CeMRI,and the association of left ventricular ejection fraction between magnetic resol3anee imaging and left ventrieulography Was also studied.Method From Junary 2005 to April 2006,27 first,onset AMI patients [23 male,mean age(54.3±10.5)years]were enrolledinthistudr.Allpatients receivedleft ventrictdographyas well as coronary angiography.The average checking time was(13.2±5.2)clays after the onset of AMI.MR imaging was performed with a 1.5-T magnet(SIMENS).After breath-hold eine images were acquired,patients re.ceived afI intravenous bolus of 0.05 mmol/kg Gd-DTPA at a rate of 5 ml/8.A first-pass perfusion scan was ac.qllired.Then a second bolus of 0.15 mmoVkg Gd-DTPA was give.at a rate of 2 mE/Is.After the hyperenhancement localized,the typical short axis slice with hyperenhancement WaS chosen to repeat imaging for IlleasuriIin.farct size every5minutesfrom5minutes after secondinjection ofcontrast until 20minutes.Results Twexty-seren patients showed hyperenhancement at the delayed CeMRI and hypoenhancement at the first pass enhancement(FPE).The average infarct size estimated by CeMRI WaS(17.9士9.8)%of LV nlass.Myocardial enhancement at a repesentative short-axis slice WIllS(7.2±6.2)%of LV Imss at 5 minutes,(8.5±7.4)%at 10 minutes,(7.3±6.3)%at 15 minutes and(6.9-t-6.4)%at 20 minutes respectively.There WltlS significant difference be-tween lmfninmes and 20-minutes enhancement size(P<0.05).Correlations of EF obtained by cineventriculo-grapIIy and MR irr,lg were significant(r=0.867,P<0.01).There were also correlations between infarction size and pe.k CK(r:O.819,P<O.01),a8 well ills peaI[cTNT(r=0.517,P<0.05)levels.Tuning of image acquisition iufluenced infarct size quantification using delayed CeMRI when TI Was kept constant.

The effects of arsenic trioxide eluting stents on expression of inflammatory factors in injured coronary arteries in pigs / 中华老年医学杂志

Objective To observe the effects of eluting stents coated with arsenic trioxide(As2O3)and suspended in poly-L-lactic acid(PLLA)on expression of monocyte chemoattractant protein-1 (MCP-1)and interleukin-6(IL-6)and to assess the effects of As2O3 eluting stents on local inflammatory reaction in injured coronary arteries in pigs. Methods Bare metal stents,rapamycin eluting stents and As2O3-eluting stents were randomly and double-blindly implanted into the anterior descending branches,circumflex branches and right coronary arteries in eight pigs.Animals were sacrificed and coronary arteries were isolated 7 days after stents implantation.The expression levels of protein and mRNA of MCP-1 and IL-6 were determined by Western blot analysis and reverse transcription polymerase chain reaction(RT-PCR),and the inflammatory cell infiltration was observed by HE staining and immunohistochemistry. Results Compared to bare metal stents,As2O3-eluting stents and rapamycin-eluting stents identically and markedly inhibited the protein expression level of MCP-1(0.421±0.055 and 0.406±0.042 vs.0.857±0.053,P＜0.01)and IL-6(0.151±0.032 and 0.146±0.051 vs.0.551±0.032,P＜0.01)and correspondingly lowered the mRNA expression level of MCP-1(0.338±0.047 and 0.327±0.051 vs.0.724±0.027,P＜0.01)and IL-6(0.531±0.052 and 0.523±0.061 vs.1.015±0.041,P＜0.01),and significantly reduced the inflammatory cell infiltration of injured coronary arteries in pigs. Conclusions As2O3-eluting stents can effectively inhibit the expressions of MCp-1 and IL-6 and reduce the inflammatory cell infiltration of injured coronary arteries in pigs.

Effect of respiration on the radiation dose distribution within target volume in radiotherapy / 中华放射医学与防护杂志

Objective To evaluate the influence of respiration on the radiation dose distribution within target volume in radiotherapy with film dosimetry. Methods Radiation of 50 MU was delivered by a square, round, ellipse, dumb bell, or female shaped filed to the films within a moving or motionless Respiration Motion Phantom respectively, the dose distributions for the two motion status were measured and compared. In order to further verify the impact of respiration, a plank phantom was used on different shifting value: 0, 0.5, 1.0, 1.5 and 2.0 cm, respectively. A square, round, or eUipse-shaped filed was used for irradiation and the distributions in different status were measured and compared with film dosimetry. Iso-dose line comparison, NAT(Normalized Agreement Tests) and γ comparison were used for the comparison of dose distributions. Fs can be an index to reflect the variability of the areas that surrounded by iso-dose lines. (FS90, FS50, FS25 delegates the ratio of the areas that surrounded by 90 %,50 %,25 % iso-dose hne in different situation respectively). Results (1) Compared with motionless situation, the FS90 in horizontal movement situation became small and the FS25 became large. As the displacement became larger, the FS90 became larger and the FS25 became smaller. FS in vertical movement situation, square and dumb bell fields changed while the others didn't have a change. (2)γ and NAT comparison: In the horizontal movement situation, compared with the static phantom, Pγ < 60 % and PNAT < 75 %. Under vertical movement situation, Pγ were less than 85 % for the square, round, dumb bell and female shaped fileds. In the plank phantom verification, Pγ and PNAT became smaller as the movement became larger. Conclusions The respiration can impact on the dose distribution within the target volume in radiotherapy, leading to a smaller area of higher dose level and an expanded area of lower dose level. The influence will become more significant with larger movement of the target.

Distribution and Resistance of AmpC Enzyme Producing Gram-negative Bacilli / 中华医院感染学杂志

OBJECTIVE To study the isolation,distributive characteristics and drug resistance of AmpC enzyme producing Gram-negative bacilli in nosocomial infection of two years and provide the evidence for treatment. METHODS A total of 528 strains of Gram-negative bacilli collected from daily specimens were identified with Bio-Fosun-Ⅰ,and AmpC enzyme was screened by cefoxitin disk and then corroborated by EDTA disk method. All data were analyzed statistically. RESULTS Among 528 strains collected,136 (25.75%) were AmpC enzyme producing strains,the respective percentage of Pseudomonas aerugionsa,Echerichia coli,Enterobacter cloacae,Klebsiella pneumoniae and Citrobacter was 32.35%,28.67%,18.38%,8.09% and 5.15%,respectively. Most strains (38.9%) were detected in ICU. The common infection sites were lungs. The resistance rate of AmpC enzyme producing strains to the first,second and third-generations cephalosporins was 71.3-99.5%. The susceptive rate of AmpC enzyme producing strains to imipenem,cefepine,amikacin and piperacillin/tazobactant were low. CONCLUSIONS For effective supervision and control of AmpC enzyme producing Gram-negative bacilli in nosocomial infection,detection of AmpC enzyme shoud be paid much attention by clinical microbiology laboratory.

Relationship between aging-induced neointima formation and Jagged1 dynamic expression in regenerating endothelial cells after arterial injury in rats / 中华老年医学杂志

ObjectiveTo investigate the relationship between aging-induced neointimal formation and Jagged 1 dynamic expression in endothelium after arterial injury in rats. MethodsForty healthy male Sprague-Dawley rats aged 3 months (young adult) and 22 months (old) were selected, and thirty of them were subjected to balloon catheter injury at the thoracic aorta. Morphometry analysis was applied to evaluate neointima/media ratio at 28 days after arterial injury. Immunohistochemistry was used to observe the dynamic expressions of Jaggedl in endothelium and the proliferating cell nuclear antigen (PCNA) in neointima at 7 days, 14 days and 28 days after arterial injury respectively. Cell co-culture system was developed by inoculating endothelial cells(EC) in the upper chamber and smooth musele eells(SMC) in the lower chamber. Fluorescence activated cell sorter (FACS) was used to assay the effect of aging on the expression of Jagged 1 in EC. ＜'3＞H-TdR incorporation and cells counting were used to determine the influence of EC of different ages of rats on platelet derived growth factor (PDGF)-induced proliferation and migration of SMC. ResultsThe neointima/media ratio were obviously higher in old rats than in young rats (0.35±0.02 vs. 0.28±0.01, n=5, P＜0.01). Compared with the young counterparts, old rats showed in immunohistochemistry that the Jaggedl in endothelium displayed a delayed up-regulation and quickly diminished evolvement pattern. The maximal enhancing level of Jaggedl in old rats was much lower than that in young ones. However, the increased extent of PCNA in neointima was significantly higher in old rats than that in young ones. Jagged 1 expression in EC in old rats was significantly lower than that in young one[(46.6±6.3)% vs. (85.4±4.0)%,n=3, P＜0.05]. The SMC co-cultured with EC in old rats exhibited higher proliferation and migration capability than those in young ones after exposure to PDGF of 10ng/ml[2H-TdR incorporation: (26 438±1857) cpm/well vs. (16 698±2076)cpm/well, n=5, P＜0.05. migration:(32±4) cells/field vs. (18±5) cells/field, n=5, P＜0. 05]ConclusionsThe up-regulation of Jaggedl in EC is impaired in aged rats, which is closely related to aging-indueed SMC proliferation and migration. It also suggests that Jagged1 might be involved in the process of aging-exaggerated neointima formation.

Respiratory effects on heart rate variability / 国际生物医学工程杂志

Respiration is one of the most important modulators of heart rate variability(HRV). The modulation mechanism of respiration on HRV were introduced. The effects of respiratory rate, respiratory volume and respiratory patterns on HRV were discussed, and these effects on HRV in patients were also referred. These respiratory effects should be taken into account when HRV is used to predict cardiac vulnerability.

Neuregulin-1 therapy improved cardiac function and reduced ANPmRNA expression in post myocardial infarction rats with cardiac dysfunction / 老年心脏病学杂志（英文版）

To observe the influence of neuregulin-1 on the cardiac function of post-myocardial infarction rats. Methods Left ventricular MI was created in Sprague-Dawley rats by ligation of the left anterior descending coronary. Six months after the operation, rats were evaluated with echocardiology methods. 36 rats that had an infarct area and a EF around 60% were randomized into 3 groups: MI group(n=12) were injected a blank vehicle fluid intravenously for 5 days, after which they continued to be raised on standard food and water for 30 days. MI+NRG group(n=12), received NRG-110μg·kg-1 intravenously for 5 days, after which they continued to be raised on standard food and water for 30 days. MI+Capt group (n=12) received captopril orally (dissolved in their drinking water 2g/L) for 30days, after which tap water substituted the solution for 5 days. Final echocardiographic and hemodynamic measurements were made at the end of 1 month of therapy. Total RNA was extracted from frozen left ventricular tissues, and was reverse transcribed into firststrand PCR was performed with primers for BNP、 ANP. Results Rats treated with neuregulin had a smaller LVDs (P=0.014), a betterLVEF (P=0.004),and a tendency towards less lung perfusion than untreated rats. Neuregulin decreased the expression of ANP mRNA in the ventricle (P=0.025).Conclusion Neuregulin markedly improved the cardiac function of rats that survived myocardial infarction,and decreased the expression of ANP mRNA in the ventricle.

Design and manufacture of mechanic modeling of fluid dynamics related to the myocardial bridging and mural coronary artery / 生物医学工程学杂志

A model of fluid dynamics related to the myocardial bridginged and mural coronary artery was designed and manufactured according to the physical principle and characteristic of the mural coronary artery. The model can imitate systematically well the effect of myocardial bridging on hemodynamic change of the mural coronary artery under different controlled experimental parameter. The methodology is proved to be feasible and has good prosperity of experimental study.

Research on Early Treatment and Evacuation of Scull Injury on Plateau / 医疗卫生装备

Field operations,work and war on plateau is an important part of future war. It is necessary to study the early treatment and evacuation of scull injury. Research and survey were made in 7 field hospitals and hundreds of records were summaried before treatment criterion in early phase was put forward for patients on plateau as well as the evacuation in different level and stage,which aims to actively save patients' lives within limited time and reduce the death rate and prevent deformity. References are provided for war,training,and construction in battlefield.

Relationship between CD117 expression and chemotherapy effect on the patients with acute nonlymphoblastic leukemia / 中国癌症杂志

Background and Purpose:It has been proved that CD117 may be used as an immunology marker for diagnosis of leukemia of myeloid origin.The relationship between CD117 expression and effect of chemotherapy on the patients with Acute Nonlymphoblastic Leukemia(ANLL) remains unclear.This study is to investigate the relationship between CD117 expression and the response of patients with ANLL to chemotherapy.Methods:Flow cytometery(FCM) was used to detect the positive rate and the levels of CD117 expression of the bone marrow mononuclear cell(BMMNC) from 38 patients with acute lymphoblastic leukemia(ALL) and 81 patients with ANLL,respectively.All-trans Retinoic Acid(ATRA) was taken to treat M_(3) type of ANLL and protocol DA and/or HA was used to treat the other types.ANLL was divided into two groups: positive(+) and negative(-) expression of CD117.At the same time we compared the difference of rates of complete remission(CR) between CD117(+) and CD117(-) groups from ANLL after chemotherapy.Results:Positive percentage of expression of CD117 in ALL and ANLL groups were 13% and 70% respectively(P=0.000).Positive levels of CD117 decreased successively as follows: M_(3)/ M_(1)、M_(2)/ M_(6) / M_(4)、M_(5).CR rates of CD117(+) and CD117(-) groups of ANLL after chemotherapy were 51%(29/57) and 67%(16/24)(P=0.192),respectively.Conclusions:CD117 may serve as an immunology marker for the diagnosis of ANLL,but positive or negative expression of CD117 in ANLL was not associated with the response of the patients with ANLL to chemotherapy.